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Enhanced expression of group II phospholipase A 2 gene in the tissues of endotoxin shock rats and its suppression by glucocorticoid
Author(s) -
Nakano Tohru,
Arita Hitoshi
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81042-m
Subject(s) - spleen , phospholipase a2 , glucocorticoid , gene expression , endocrinology , medicine , in vivo , kidney , messenger rna , biology , lipopolysaccharide , chemistry , microbiology and biotechnology , gene , immunology , biochemistry , enzyme
We studied the regulation of group II phospholipase A 2 (PLA 2 ‐II) gene in vivo, using endotoxin shock rat as a model for systemic inflammation. Administration of endotoxin into rats increased PLA 2 activity in the plasma, as described by Vadas and Hay, using endotoxin‐challenged rabbit. Specific absorption of this activity by anti‐PLA 2 ‐II antibody indicated that the released PLA 2 was PLA 2 ‐II. The levels of PLA 2 ‐II mRNA were elevated in the aorta, spleen, lung, and thymus but not in the liver and kidney. The tissues with high PLA 2 ‐II mRNA contents released a greater amount of PLA 2 ‐II than the tissues of control rats. These results suggest that in endotoxin shock rats, PLA 2 ‐II is synthesized de novo in the above tissues and released into circulation. Furthermore, our present study demonstrates that glucocorticoid suppresses the enhanced expression of the PLA 2 ‐II gene in the tissues of endotoxin shock rats.