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Inhibition of development of Na + ‐dependent hexose transport in renal epithelial LLC‐PK1 cells by differentiation‐stimulating factor for myeloid leukemic cells/leukemia inhibitory factor
Author(s) -
Tomida Mikio,
Yamamoto-Yamaguchi Yuri,
Hozumi Motoo,
Holmes William,
Lowe David G.,
Goeddel David V.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81023-h
Subject(s) - leukemia inhibitory factor , growth factor , myeloid leukemia , cytokine , cell culture , chemistry , biology , microbiology and biotechnology , cellular differentiation , biochemistry , receptor , immunology , interleukin 6 , genetics , gene
Differentiation‐stimulating factor (D‐factor)/leukemia inhibitory factor is a cytokine inducing differentiation of mouse myeloid leukemie M1‐T22 cells. The effect of recombinant human D‐factor on growth and differentiation of pig kidney LLC‐PK 1 cells was examined. LLC‐PK 1 cells did not concentrate α‐methylglucoside during their early growth in culture but developed the capacity to concentrate this hexose as they reached confluence and their growth rate decreased. Purified D‐factor caused dose‐dependent inhibition of the development of this concentrative capacity. It did not affect the growth rate of the cells, but inhibited the formation of multicellular domes in confluent cultures. LLC‐PK, cells were found to have high‐affinity binding sites (831 per cell) for D‐factor with a dissociation constant of 197 pM.