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N ‐Furfurylformamide as a pseudo‐substrate for formylmethanofuran converting enzymes from methanogenic bacteria
Author(s) -
Breitung J.,
Börner G.,
Karrasch M.,
Berkessel A.,
Thauer R.K.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81022-g
Subject(s) - bacteria , enzyme , substrate (aquarium) , chemistry , biochemistry , microbiology and biotechnology , biology , ecology , genetics
Methanofuran involved in methanogenesis. The N ‐formyl derivative is an intermediate in the reduction of CO 2 to CH 4 and the disproportionation of methanol to CO 2 and CH 4 . Formylmethanofuran dehydrogenase and formylmethanofuran:tetrahydromethanopterin formyltransferase are the enzymes catalyzing its conversions. We report here that the two enzymes from Methanosarcma barkeri and the formyltransferase from Methanobacterium thermoautotrophicum can also use N ‐furfurylfonnamide as a pseudo‐substrate albeit with higher apparent K m and lower apparent V max values. N ‐Methylformamide, formamide, and formate were not converted indicating that the furfurylamine moiety of methanofuran is the minimum structure required for the correct binding of the coenzyme.