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RNA editing of ATPase subunit 9 transcripts in Oenothera mitochondria
Author(s) -
Schuster Wolfgang,
Brennicke Axel
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81021-f
Subject(s) - biology , oenothera , open reading frame , genetics , rna editing , start codon , microbiology and biotechnology , mitochondrial dna , amino acid , stop codon , protein subunit , complementary dna , messenger rna , serine , population , biochemistry , gene , peptide sequence , enzyme , demography , sociology
The mRNA for subunit 9 of the ATPase (atp9) in the higher plant Oenothera is edited in four nucleotide positions. Three events alter genomic serine and proline codons to triplets specifying leucine. A UGA termination codon is introduced into the reading frame by modification of a CGA arginine codon. This modification shortens the polypeptide by four amino acids. Direct sequencing of PCR amplified cDNA from the total mitochondrial mRNA population gives no indication of partially edited transcripts suggesting a rapid and efficient modification of atp9 transcripts in Oenothera mitochondria.