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The effect of adenosine on the fluorescence responses of chlorotetracycline‐loaded human polymorphonuclear leukocytes
Author(s) -
Tsuruta Satoru,
Ito Setsuko,
Mikawa Haruki
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81018-j
Subject(s) - adenosine , adenosine deaminase , adenosine receptor , chemistry , purinergic signalling , chemotaxis , extracellular , adenosine a1 receptor , adenosine a3 receptor , theophylline , calcium , adenosine receptor antagonist , receptor , n formylmethionine leucyl phenylalanine , biochemistry , pharmacology , biology , agonist , organic chemistry
Chlorotetracycline has been used in human polymorphonuclear leukocytes as a probe to investigate the state of membrane‐bound calcium. We examined the effect of adenosine on the fluorescence responses of CTC‐loaded PMNs stimulated with the synthetic chemotactic peptide, formylmethionyl‐leucyl‐phenylalanine. Adenosine inhibited the decrease in CTC fluorescence in a dose‐dependent fashion and its effect was reversed by theophylline, an adenosine receptor antagonist. Removal of extracellular adenosine by incubating PMNs with adenosine deaminase abolished the effect of adenosine. These data suggest that adenosine inhibits the release of membrane‐bound calcium in PMNs that normally occurs in response to chemotactic stimuli, acting via PMN surface adenosine receptors.