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Fixation of a highly reactive form of α‐chymotrypsin by micellar matrix
Author(s) -
Levashov A.V.,
Klyachko N.L.,
Bogdanova N.G.,
Martinek K.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81017-i
Subject(s) - chymotrypsin , chemistry , micelle , reactivity (psychology) , matrix (chemical analysis) , chromatography , polymer chemistry , enzyme , organic chemistry , trypsin , aqueous solution , medicine , alternative medicine , pathology
Using reversed micelles of surfactants solvated by water‐organic cosolvent mixtures as a matrix for enzyme entrapping, it is possible to fix the highly reactive α‐chymotrypsin form. The reactivity of α‐chymotrypsin towards nonspecific substrates increases to the extent comparable with that observed in reactions involving specific substrates.