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Human chorionic gonadotropin α and human cytomegalovirus promoters are extremely active in the fission yeast Schizosaccharomyces pombe
Author(s) -
Toyama Reiko,
Okayama Hiroto
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81012-d
Subject(s) - schizosaccharomyces pombe , yeast , human chorionic gonadotropin , schizosaccharomyces , fission , chemistry , biology , biochemistry , physics , saccharomyces cerevisiae , hormone , quantum mechanics , neutron
We have investigated the transcriptional activity of human cytomegalovirus, herpes thymidine kinase, human chorionic gonadotropin α, somatostatin, immunoglobulin κ chain, α crystallin, albumin and interferon‐β promoters in the fission yeast Schizosaccharomyces pombe . Among these, the human cytomegalovirus, human chorionic gonadotropin α, and somatostatin promoters were found to be very active, approximately 11‐, 9‐, and 0.9‐fold as active as the SV40 early promoter, respectively. The remainder of the promoters studied were weak, having only 10–20% of the SV40 promoter activity. Primer extension analysis showed that the strong promoters initiated transcription in S. pombe at the same sites as in mammalian cells, indicating the high similarity between both transcriptional systems.