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Phosphotyrosine‐containing proteins in Dictyostelium discoideum
Author(s) -
Schweiger Anton,
Mihalache Oana,
Muhr Angelika,
Adrian Iris
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81007-b
Subject(s) - dictyostelium discoideum , immunoprecipitation , monoclonal antibody , tyrosine , phosphorylation , biochemistry , microbiology and biotechnology , dictyostelium , antibody , mycetozoa , chemistry , biology , gene , immunology
Phosphotyrosine‐containing proteins in Dictyostelium discoideum were detected by immunoblot analysis and inununoprecipitation using a monoclonal anti‐phosphotyrosine antibody. The iodinated antibody recognized on bots a cluster of 205–220 kDa polypeptides and bands of 107 and 60 kDa. The 107 and 60 kDa polypeptides and, in addition, a 82 kDa one became phosphorylated on tyrosine when the immunoprecipitate was incubated with [γ‐ 32 p]ATP. In preparations from differentiating cells the intensity of the label was increased in the 60 kDa band and decreased in the 107 and 205–220 kDa bands.