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Localization of aldolase C mRNA in brain cells
Author(s) -
Popovici Théodora,
Berwald-Netter Yoheved,
Vibert Magdeleine,
Kahn Axel,
Skala Henriette
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)81005-9
Subject(s) - aldolase a , in situ hybridization , messenger rna , striatum , biology , northern blot , microbiology and biotechnology , fructose bisphosphate aldolase , cerebral cortex , cortex (anatomy) , biochemistry , enzyme , neuroscience , gene , dopamine
The expression of aldolase C and aldolase A mRNA was assessed by Northern blot hybridization using RNAs purified from cultured rat and mouse brain neurons and astroglial cells. Neurons were found to contain about 4‐fold more aldolase C mRNA and about twice as much aldolase A mRNA than astroglia. Analysis of the cellular localization of aldolase C mRNA by in situ hybridization to brain slices showed a predominantly neuronal labeling with an irregular distribution. A strong signal was observed in Purkinje cell somata and a weaker signal in subpopulations of neurons in cerebral cortex, striatum, hippocampus, hypothalamic nuclei and primary olfactory cortex.