Premium
Superinduction of ornithine decarboxylase (ODC) by actinomycin D is due to stimulation of ODC mRNA translation
Author(s) -
Wallon U.Margaretha,
Persson Lo,
Heby Olle
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80998-x
Subject(s) - cycloheximide , ornithine decarboxylase , dactinomycin , messenger rna , protein biosynthesis , ornithine decarboxylase antizyme , stimulation , microbiology and biotechnology , biology , chemistry , biochemistry , endocrinology , enzyme , gene
Inhibition of transcription by treatment with actinomycin D caused superinduction of the ornithine decarboxylase (ODC) activity in Ehrlich ascites tumor cells. Experiments with cycloheximide ruled out the possibility that this superinduction was due to stabilization of ODC. Instead the ODC activity exhibited a more rapid turnover in the presence of actinomycin D ( t=36 min) than in its absence ( t= 56 min). The superinduction was found to coincide with an increased rate of ODC synthesis, as determined by measuring the incorporation of [ 35 S]methionine into immunoreactive ODC protein. The steady‐state level of ODC mRNA was unchanged, indicating an effect on the translational efficiency.