Premium
Endothelin stimulates hypertrophy and contractility of neonatal rat cardiac myocytes in a serum‐free medium
Author(s) -
Suzuki Takahiko,
Hoshi Hiroyoshi,
Mitsui Youji
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80995-u
Subject(s) - contractility , myocyte , medicine , endocrinology , cardiac myocyte , muscle hypertrophy , endothelin 1 , endothelin receptor , nicardipine , chemistry , protein kinase c , calcium , extracellular , biology , kinase , microbiology and biotechnology , receptor
The effect of endothelin (ET) on rat cardiac myocytes cultured in a serum‐free, defined medium was determined. ET stimulated cardiac myocyte hypertrophy in a dose‐dependent manner as determined by the protein synthesis and cell surface area. Since the myocyte hypertrophy was abolished by H‐7, a protein kinase C inhibitor, ET‐receptor mediated protein kinase C activation may be involved in cardiac myocyte hypertrophy. At the same time, ET also stimulated myocyte contractility in this medium, and this stimulatory effect was inhibited by nicardipine. This result indicates that the influx of extracellular calcium ion is necessary for the stimulation of contractility induced by ET.