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Exacerbation of rat adjuvant arthritis by intradermal injection of purified mammalian 14‐kDa group II phospholipase A 2
Author(s) -
Murakami Makoto,
Kudo Ichiro,
Nakamura Hideo,
Yokoyama Yuichi,
Mori Hiroshi,
Inoue Keizo
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80986-s
Subject(s) - phospholipase a2 , intradermal injection , edema , pathogenesis , arthritis , inflammation , carrageenan , phospholipase a , adjuvant , chemistry , phospholipase , immunology , pharmacology , platelet , medicine , exacerbation , endocrinology , enzyme , biochemistry
Appreciable phospholipase A 2 activity was detected in a hind paw homogenate from rats with adjuvant arthritis or carrageenan‐induced edema. The activity was neutralized by treatment with antibody raised against rat platelet secretory phospholipase A 2 , indicating that 14‐kDa group II phospholipase A 2 , was induced in the hind paw during the process of inflammation. Injection of purified rat platelet phospholipase A 2 into the hind paw of rats with adjuvant‐induced arthritis resulted in exacerbation of edema in a dose‐dependent manner, whereas no effect was observed on either normal rats or animals with carrageenan‐induced edema under the same experimental conditions. These results suggest the importance of mammalian group II phospholipase A 2 , in the pathogenesis of some types of inflammation.