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Estimation of adenosine triphosphate utilization of rat mast cells during and after anaphylactic histamine secretion
Author(s) -
Johansen Torben
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80983-p
Subject(s) - histamine , adenosine triphosphate , secretion , oxidative phosphorylation , chemistry , adenosine , mast cell , adenosine diphosphate , medicine , endocrinology , biochemistry , biology , immunology , platelet , platelet aggregation
Determination of the cellular content of adenosine triphosphate (ATP) and the rate of ATP‐synthesis were used to estimate the cellular utilization ofATP in relation to anaphylactic histamine secretion. There was an increased rate of oxidative ATP‐synthesis and a decreased cellular ATP content during the time period of histamine secretion and immediately after its completion. During secretion the additional ATP‐utilization above the basal level of ATP‐synthesis was 0.51cells. 2.5 min after cell activation, the rate of additional ATP‐utilization was 0.30, and the persistent ATP‐decrease observed after 30 and 120 min may be due to a decreased rate of oxidative ATP‐synthesis.