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3‐Azido‐L‐tyrosine as a photoinhibitor of tubulin:tyrosine ligase
Author(s) -
Coudijzer Katleen,
Joniau Marcel
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80981-n
Subject(s) - tyrosine , dna ligase , chemistry , phenylalanine , biochemistry , enzyme , tubulin , biology , amino acid , microtubule , microbiology and biotechnology
We have synthesized the photoactivatable probes 3‐azido‐L‐tyrosine and p ‐azido‐L‐phenylalanine and studied their capacity to inhibit the incorporation of[ 3 H]tyrosine into tubulin catalyzed by tubulin:tyrosine ligase. Without illumination, only 3‐azido‐L‐tyrosine reversibly inhibits the enzyme. Upon illumination, both reagents irreversibly photoinactivate the enzyme in a similar way. The ligase can be protected against photoinactivation by reversibly blocking essential thiol groups with pCMB during illumination.