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The cholecystokinin gene is abundantly co‐expressed with gastrin‐releasing peptide, enkephalin and neuropeptide Y genes in a clonal human neuroepithelioma cell line
Author(s) -
Verbeeck Marleen A.E.,
Burbach J.Peter H.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80979-s
Subject(s) - cholecystokinin , gastrin , neuropeptide , enkephalin , neuropeptide y receptor , gastrin releasing peptide , gene , peptide , biology , microbiology and biotechnology , endocrinology , biochemistry , receptor , secretion , bombesin , opioid
The cholinergic human neuroepithelioma cell line SK‐N‐MCIXC expressed mRNAs for the neuropeptides cholecystokinin (CCK), neuropeptide Y, gastrin‐releasing peptide (GRP) and enkephalin. The CCK transcript of about 800 nt was present at very high levels and CCK‐like peptides immunoreactive to a C‐terminal CCK octapeptide antiserum were present in the cell line and its medium. This clonal neuronal cell line provides a unique model system to identify cis ‐ and trans‐acting factors responsible for neuron‐specific expression and regulation of the CCK gene. Further‐more, the pluripotent properties of the undifierentiated cell line may open studies on neuronal differentiation at the level of co‐expression of neuropeptides and transmitters.