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Rapid and reversible changes in myosin heavy chain expression in response to increased neuromuscular activity of rat fast‐twitch muscle
Author(s) -
Kirschbaum Bernhard J.,
Schneider Susanne,
Izumo Seigo,
Mahdavi Vijak,
Nadal-Ginard Bernardo,
Pette Dirk
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80976-p
Subject(s) - myosin , messenger rna , gene isoform , stimulation , gene expression , endocrinology , medicine , biology , chemistry , microbiology and biotechnology , gene , biochemistry
Chronic 10 Hz stimulation of rat fast‐twitch muscle induced rapid and reversible changes in the tissue levels offast myosin heavy chain (HC) mRNA isoforms. These changes consisted of a rapid decrease in HCIIb mRNA and a progressive increase in HCIIa mRNA. After 15 days, the HCIIb mRNA normally amounting to approximately 80%, had decreased to less than 5% of the sum of the two HC mRNA isoforms. HCIIb mRNA was again detectable one day after cessation of stimulation and progressively increased at the expense of HCIIa mRNA with ongoing recovery. These results point to a down‐regulation of the HCIIb gene by the applied stimulus pattern which, conversely, enhances the expression of the HCIIa gene.