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A novel TIMP‐insensitive type IV collagen‐degrading metalloproteinase from murine metastatic sarcoma cells
Author(s) -
Kato Yoichi,
Ogawa Kohei,
Yamamoto Shuji,
Abe Shigeto,
Kishi Jun-ichi,
Hayakawa Taro
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80967-n
Subject(s) - matrix metalloproteinase , metalloproteinase , enzyme , trypsin , chemistry , biochemistry , microbiology and biotechnology , type i collagen , cell culture , biology , endocrinology , genetics
A novel type IV collagen‐degrading metalloproteinase was purified from the conditioned media of a murine metastatic sarcoma cell line. The molecular weight of the purified enzyme was determined to be 100 kDa by SDS‐PAGE, while 700 kDa by gel filtration suggesting that the enzyme has a multimer structure. This enzyme degrades type IV collagen, but neither type I collagen nor casein. The failure of trypsin treatment to enhance the enzyme activity suggested that the purified enzyme did not require activation. Although the enzyme seems to be classified as a matrix metalloproteinase, it was inhibited by neither tissue inhibitor of metalloproteinases (TIMP) nor TIMP‐2 and thus represents a novel type IV collagen‐degrading metalloproteinase.