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Grapevine stilbene synthase cDNA only slightly differing from chalcone synthase cDNA is expressed in Escherichia coli into a catalytically active enzyme
Author(s) -
Melchior Frauke,
Kindl Helmut
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80961-h
Subject(s) - chalcone synthase , complementary dna , escherichia coli , atp synthase , biochemistry , enzyme , biology , microbiology and biotechnology , chemistry , biosynthesis , gene
Stilbene synthase is responsible for the formation of resveratrol and other stilbenes which function in grapevine as phytoalexins. A full‐length stilbene synthase cDNA was prepared from grapevine mRNA and sequenced. The insert in pSV25 coding for a polypeptide with 392 amino acids was inserted into the vectors pKK233‐2 and pDS12/RBSII‐2, respectively. Expression of the cDNA in Escherichia coli yielded an enzyniatically active dimer exhibiting solely stilbene synthase activity. The protein was characterized by enzyme activity and Western blot analysis.