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Differential sensitivity of bicarbonate‐reversible formate effects on herbicide‐resistant mutants of Synechocystis 6714
Author(s) -
Vernotte Claudie,
Peteri Brigitta,
Astier Chantal,
Etienne Anne-Lise
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80943-d
Subject(s) - mutant , dcmu , biology , formate , bicarbonate , synechocystis , biochemistry , chlorophyll , bioluminescence , biophysics , photosystem ii , botany , photosynthesis , gene , endocrinology , catalysis
Herbicide‐resistant mutants of the cyanobacterium Synechocystis 6714, that are altered in specific amino acids in their D‐l protein, show differential sensitivity to formate treatment. Measurements on oxygen yield in a sequence of flashes, chlorophyll (Chl) a fluorescence transients and Chl a fluorescence yield decay after a flash reveal that the resistance of cells to formate treatment is in the following (highest to lowest) order: [double mutant] A251V/F211S (Az V) > [single mutant] F211S (Az I) ⋍wild type > [single mutant] S264A (DCMU II‐A). Significance of these results in terms of overlapping between the herbicide and bicarbonate binding niches on the D‐I protein is discussed.