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Apis mellifera cytoplasmic elongation factor 1α (EF‐1α) is closely related to Drosophila melanogaster EF‐1α
Author(s) -
Walldorf Uwe,
Hovemann Bernhard T.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80936-d
Subject(s) - drosophila melanogaster , biology , melanogaster , genetics , open reading frame , elongation factor , gene , nucleic acid sequence , clone (java method) , genomic dna , drosophila (subgenus) , peptide sequence , microbiology and biotechnology , ribosome , rna
Using low stringency hybridisation with a Drosophila melanogaster EF‐1α gene fragment we have isolated a genomic DNA clone encoding elongation factor 1α (EF‐1α) from Apis mellifera . The hybridising Apis mellifera sequence could be delineated to two small Eco RI fragments that were also revealed by genomic Southern hybridisation. By comparison with the corresponding Drosophila melanogaster data the complete translational reading frame has been deduced. It is interrupted by two intervening sequences of 220 and about 790 nucleotides. Comparison with known eucaryotic EF‐1α sequences further confirms that certain amino acid sequences seem to be invariable within the EF‐1α protein family.