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Coupling of early response gene expression to distinct regulatory pathways during α‐interferon and phorbol ester‐induced plasmacytoid differentiation of B chronic lymphocytic leukaemia cells
Author(s) -
Murphy John J.,
Norton John D.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80935-c
Subject(s) - signal transduction , phorbol , biology , cellular differentiation , regulation of gene expression , interleukin 4 , interferon regulatory factors , gene expression , interferon , microbiology and biotechnology , gene , chemistry , immunology , cytokine , transcription factor , biochemistry , protein kinase c
Phorbol esters (phorbol 12‐myristate 13‐acetate; PMA) and α‐interferon (α‐IFN) act through divergent signal transduction pathways to induce B chronic lymphocytic leukaemia cells (B‐CLL) to undergo plasmacytoid differentiation in vitro. By using a panel of PMA‐inducible early response gene probes we show that these two different effectors are coupled to second messenger pathways that do not converge on a common gene regulatory programme in differentiation of B‐CLL cells. Moreover, using the calcium ionophore, A23187, four distinct regulatory classes of early response gene could be defined implying that multiple regulatory pathways may mediate the process of terminal differentiation in B lymphocytes.