Premium
The involvement of fatty acid binding protein in peroxisomal fatty acid oxidation
Author(s) -
Reubsaet F.A.G.,
Veerkamp J.H.,
Brückwilder M.L.P.,
Trijbels J.M.F.,
Monnens L.A.H.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80931-8
Subject(s) - peroxisome , biochemistry , fatty acid binding protein , fatty acid , palmitic acid , organelle , free fatty acid receptor , oxidase test , beta oxidation , chemistry , mitochondrion , peroxisomal targeting signal , phytanic acid , adipocyte protein 2 , mixed function oxidase , enzyme , polyunsaturated fatty acid , microsome , receptor , gene
Rat liver fatty acid‐binding protein (FABP) can function as a fatty acid donor protein for both peroxisomal and mitochondrial fatty acid oxidation, since 14 C‐labeled palmitic acid bound to FABP is oxidized by both organelles. FABP is, however, not detected in peroxisomes and mitochondria of rat liver by ELISA. Acyl‐CoA oxidase activity of isolated peroxisomes was not changed by addition of FABP or flavaspidic acid, an inhibitor of fatty acid binding to FABP, nor by disruption of the peroxisomal membranes. These data indicate that FABP may transfer fatty acids to peroxisomes, but is not involved in the transport of acyl‐CoA through the peroxisomal membrane.