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Cooperativity between platelet‐activating factor and collagen in aggregation of bovine platelets III
Author(s) -
Kojima Soichi,
Sekiya Fujio,
Inada Yuji,
Sato Fumie,
Tsukada Toshiyasu,
Saito Yuji
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80930-h
Subject(s) - chemistry , phosphatidic acid , platelet , platelet activating factor , arachidonic acid , antagonist , thromboxane , platelet aggregation , cooperativity , biophysics , biochemistry , microbiology and biotechnology , receptor , endocrinology , medicine , biology , phospholipid , membrane , enzyme
In cooperative aggregation of bovine platelets induced by simultaneous addition of PAF and collagen at subthreshold concentrations the following observations were made. (i) Formation of phosphatidic acid and arachidonic acid metabolites, which characterize PAF and collagen alone‐induced aggregation, respectively, was observed very obviously. (ii) A thromboxane antagonist did not completely block the cooperative aggregation. The extent of residual aggregation activity was dependent on concentration of collagen used in the simultaneous administration with PAF. These results suggest that both signal transduction pathways activated by PAF and collagen alone at high concentrations are attained by simultaneous addition of both agonists at subthreshold concentrations through unknown mechanisms.