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Human c‐myc gene contains a regulatory site similar to consensus of interferon response sequence (IRS)
Author(s) -
Alexandrova N.M.,
Itkes A.V.,
Imamova L.R.,
Chernov B.K.,
Tulchinsky E.M.,
Ulyanov N.B.,
Kisselev L.L.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80885-m
Subject(s) - intron , consensus sequence , gene , biology , transcription (linguistics) , microbiology and biotechnology , oligonucleotide , regulatory sequence , transcription factor , binding site , gata6 , genetics , peptide sequence , linguistics , philosophy
Expression of c‐myc proto oncogene is regulated by multiple mechanisms. Here, we report that the consensus of the regulatory region of interferondependent genes, GGAAAN 1−3 GAAA, was found after computer search in the 5'‐terminal flank of human c‐myc gene in position (−76:−67). In vitro transcription of c‐myc gene fragments showed that the consensus region competes with oligonucleotide GGGAAAATGAAACT for binding to specific protein(s). This oligonucleotide was shown to bind selectively the interferon‐dependent positive transcription factor [1]. Transcription of c‐myc fragments lacking 5'‐terminal region up to positions −101 or +71 was initiated at two sites located in the first intron. These sites did not coincide with P 1 in vivo RNA cap‐site. Binding of the protein factor(s) to the regulatory region of c‐myc gene −76:−67 blocked the in vitro transcription initiated in the first intron.