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Functional expression of the mutants of the chloroplast tRNA Lys gene from the liverwort, Marchantia polymorpha , in Escherichia coli
Author(s) -
Nakahigashi Kenji,
Inokuchi Hachiro,
Ozeki Haruo
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80883-k
Subject(s) - marchantia polymorpha , escherichia coli , mutant , transfer rna , biology , gene , chloroplast , genetics , microbiology and biotechnology , rna
The anticodon of the tRNA Lys gene (trnK) in the liverwort, Marchantia polymorpha , was anificially converted to an amber anticodon. This mutant tRNA Lys (CTA) gene carrying either the intron or the C 27 ‐C 43 mismatch at the anticodon‐stem is not functional in Escherichia coli , but without both of them, it does work as a tRNA Lys amber suppressor.