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Anion binding to the chloride pump, halorhodopsin, and its implications for the transport mechanism
Author(s) -
Lanyi Janos K.,
Duschi Albert,
Váro György,
Zimányi László
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80869-k
Subject(s) - halorhodopsin , halobacteriaceae , chemistry , chloride , ion transporter , biophysics , binding site , kinetics , ion , membrane , biochemistry , biology , bacteriorhodopsin , halobacterium salinarum , organic chemistry , physics , quantum mechanics
The light‐driven chloride pump, halorhodopsin, binds and transports chloride across the membrane, and to a lesser extent nitrate. Binding and transport kinetics, and resonance Raman spectra of the retinal Schiff base, with these anions suggest the existence of two mutually exclusive binding sites. One of these may be the uptake site, and the other the release site during the transport. Plausible locations can be suggested for these sites, because halorhodopsin is a small protein with few buried positively charged residues, and the primary structure of a second pigment with similar function has recently become available for comparison.