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Oxidized low‐density lipoprotein is chemotactic for arterial smooth muscle cells in culture
Author(s) -
Autio Ismo,
Jaakkola Olli,
Solakivi Tiina,
Nikkari Tapio
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80857-f
Subject(s) - chemotaxis , agarose , low density lipoprotein , chemistry , smooth muscle , stimulation , agarose gel electrophoresis , lipoprotein , biochemistry , gel electrophoresis , medicine , endocrinology , cholesterol , biology , receptor , dna
The effects of human native and Cu 2+ ‐oxidized low‐density lipoprotein (LDL) were tested on the migration of cultured bovine aortic smooth muscle cells (SMCs) in blind‐well chambers. LDL oxidation was controlled by measuring the formation of conjugated dienes and lipid hydroperoxides, and by agarose gel electrophoresis. Oxidized LDL stimulated SMC migration, and the effect was dose‐dependent up to 200 . The stimulation was chemotactic in nature. Native LDL was without significant activity. The results suggest that oxidized LDL may contribute to the migration of medial SMCs into the intima during atherogenesis.