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Inositol 1,4,5‐trisphosphate activates two types of Ca 2+ ‐permeable channels in human carcinoma cells
Author(s) -
Mozhayeva Gali.,
Naumov Alexander P.,
Kuryshev Yuri A.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80853-b
Subject(s) - extracellular , inositol , biophysics , patch clamp , chemistry , pipette , intracellular , membrane , inositol trisphosphate , ion channel , biochemistry , receptor , biology
Ca 2+ ‐permeable channels in human carcinoma A431 cells were studied using the patch clamp technique. We have found two types of Ca 2+ ‐penneable channels which are activated by inositol 1,4,5‐trisphosphate (IP 3 ) applied to the intracellular side of the plasma membrane. Unitary conductances of these channels are 3.7 and 13 pS (105 mM Ca 2+ in recording pipette, 30–33°C). From the extracellular side of the membrane the channels are activated by EGF. It is assumed that extracellular agonists open both channel types by stimulating the release of IP 3 from the membrane.