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Nuclear factor‐1 (NF‐1) binds to multiple sites within the transcriptional enhancer of Moloney Murine Leukemia virus
Author(s) -
Reisman David
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80846-b
Subject(s) - enhancer , murine leukemia virus , transcription factor , biology , enhancer rnas , transcription (linguistics) , binding site , microbiology and biotechnology , tropism , virus , virology , gene , genetics , linguistics , philosophy
The transcriptional enhancer of the Moloney Murine Leukemia virus (Mo‐MuLV) is comprised of a 75‐bp direct repeat, each of which contains multiple binding sites for transcription factors. The occupancy of these sites determines the tissue specificity of expression and disease tropism of the virus. The identification of proteins that bind to this enhancer is therefore required in order to understand the molecular basis of this viral‐host interaction. Analysis of the nucleic acid sequence of the Mo‐MuLV has identified 4 potential binding sites for the transcription factor NF‐1. Evidence is presented using DNase I protection analysis that NF‐1 binds to these 4 sites within the enhancer. The potential role of NF‐1 binding in tissue specific expression of Mo‐MuLV is discussed.