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Immunoelectron microscopical localization of the catalytic subunit of cAMP‐dependent protein kinases in brain microtubules and neurofilaments
Author(s) -
Trinczek Bernhard,
Schwoch Gerhild
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80835-7
Subject(s) - neurofilament , immunogold labelling , microtubule , protein subunit , immunoelectron microscopy , microbiology and biotechnology , microtubule associated protein , kinase , biology , protein kinase a , chemistry , biochemistry , ultrastructure , anatomy , immunohistochemistry , immunology , gene
The catalytic subunit of cAMP‐dependent protein kinases was localized in microtubules and neurofilaments by immunogold electron microscopy. In microtubules, the label was similarly distributed as an immunolabel for the microtubule associated protein MAP 2. The neurofilaments showed no reaction with the MAP 2‐antiserum. Our results support the suggestion of an in vivo role of cAMP‐dependent protein kinases in the regulation of microtubules. In addition, this is the first demonstration that cAMP‐dependent protein kinase is associated with neurofilaments.