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Studies on the primary sequence requirements for PKC‐α, ‐β 1 and ‐γ peptide substrates
Author(s) -
Marais Richard M.,
Nguyen Oanh,
Woodgett James R.,
Parker Peter J.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80831-3
Subject(s) - peptide , primary (astronomy) , sequence (biology) , protein primary structure , peptide sequence , protein kinase c , chemistry , biochemistry , computational biology , combinatorial chemistry , biology , kinase , gene , physics , astronomy
The substrate specificity of purified PKC‐α, ‐β and ‐γ has been investigated. A series of synthetic peptides based upon the sequence surrounding serine‐7 in glycogen synthase were generated and used to determine the basic residue requirements of these PKC isotypes. While PKC‐α and ‐β are indistinguishable in their phosphorylation of these peptides, PKC‐γ shows a distinct specificity profile for these synthetic substrates.