Premium
Sequence analysis of the potent mitogenic toxin of Pasteurella multocida
Author(s) -
Lax Alistair J.,
Chanter Neil,
Pullinger Gillian D.,
Higgins Theresa,
Staddon James M.,
Rozengurt Enrique
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80809-w
Subject(s) - pasteurella multocida , toxin , microbiology and biotechnology , sequence (biology) , sequence analysis , biology , chemistry , genetics , bacteria , gene
Pasteurella multocida toxin is a potent mitogen for cultured Swiss 3T3 cells where it causes an accumulation of inositol phosphates and activation of protein kinase C. The gene sequence described here coded for a 146 kDa protein. The ORF was preceded by a ribosome binding site and followed by a stem loop. There was no evidence for a signal sequence. The gene had a low G + C base ratio which differs from the rest of the Pasteurella genome. There was no significant homology with other known proteins, although a motif found in certain bacterial toxins which are ADP‐ribosyl transferases is present. A recombinant expressing only part of the PMT gene was not mitogenic.