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Correlation between endotoxin‐neutralizing capacity of human plasma as tested by the limulus‐amebocyte‐lysate‐test and plasma protein levels
Author(s) -
Berger D.,
Schleich S.,
Scidelmann M.,
Beger H.G.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80803-q
Subject(s) - limulus amebocyte lysate , limulus , lysis , human plasma , chemistry , chromatography , biology , biochemistry , immunology , lipopolysaccharide , paleontology
In the present study the endotoxin‐neutralizing capacity of human plasma obtained from healthy volunteers was determined by use of the limulus‐amebocyte‐lysate test. The extent of the endotoxin‐neutralizing capacity which showed a very broad variation was correlated to the plasma levels of some proteins which are believed to contribute to endotoxin transport and detoxification. The plasma levels of α 2 ‐macroglobulin and transferrin, the major transport proteins, as well as the levels of IgG, IgA and IgM and apoprotein A were not significantly correlated to the neutralizing capacity. Only the apoprotein B, the major apoprotein of the low density lipoprotein fraction shows a significant correlation. Together with previously published results it should be concluded that low density lipoproteins are involved in the endogenous endotoxin‐neutralizing reaction occuring in human plasma, at least when high doses of endotoxin are administered. Nevertheless the endotoxinneutralizing reaction seems to be a very complex multistep process.