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Stimulation of actin synthesis in phalloidin‐treated cells
Author(s) -
Serpinskaya A.S.,
Denisenko O.N.,
Gelfand V.I.,
Bershadsky A.D.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80797-m
Subject(s) - phalloidin , microfilament , actin , cytoskeleton , microbiology and biotechnology , stimulation , chemistry , biology , cell , biochemistry , endocrinology
We used the technique of scrape loading to introduce phalloidin into mouse embryo fibroblasts in mass culture. Phalloidin almost completely destroyed actin microfilament bundles, but the amount of polymerized cytoskeleton‐associated actin was increased approximately two‐fold and the amount of monomeric (Triton X‐100 extractable) actin was significantly reduced. The major result of the present study is that the rate of actin synthesis in the phalloidin‐treated cells was 2–3 times higher than in the control cells. Northern blot and translation in a cell‐free system from rabbit reticulocytes showed that the actin mRNA level significantly increased as a result of phalloidin treatment.