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Interaction of puromycin with acceptor site of human placenta 80 S ribosomes
Author(s) -
Graifer D.M.,
Fedorova O.S.,
Karpova G.G.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80795-k
Subject(s) - puromycin , ribosome , chemistry , acceptor , translation (biology) , placenta , transfer rna , human placenta , biochemistry , stereochemistry , protein biosynthesis , biology , messenger rna , fetus , rna , pregnancy , genetics , physics , condensed matter physics , gene
The complex N ‐AcPhe‐tRNA Phe · poly(U) · 80 S ribosome from human placenta was treated with puromycin taken in various concentrations. Based on the kinetic data of N ‐acetylphenylalanyl‐puromycin formation, the association constant of puromycin with the acceptor site of the ribosome was estimated to be (3.96 ± 0.84) × 10 4 M −1 at 37°C.