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A 36‐kDa mitochondrial protein is responsible for cyanide‐resistant respiration in Hansenula anomala
Author(s) -
Minagawa Nobuko,
Sakajo Shigeru,
Komiyama Tadazumi,
Yoshimoto Akio
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80787-j
Subject(s) - cyanide , antimycin a , respiration , biochemistry , mitochondrion , biology , protein biosynthesis , chemistry , botany , inorganic chemistry
Antimycin A‐dependent induction of cyanide‐resistant respiration in Hansenula anomala was reversibly blocked by carbonylcyanide‐ m ‐chlorophenylhydrazone (CCCP). When the cells were pulse‐labeled with [ 35 S]methionine in the presence of both antimycin A and CCCP, the radioactivity was incorporated into a 39 kDa mitochondrial protein. Upon removal of CCCP, this protein was processed into a 36 kDa form. The increase in the 36 kDa protein completely paralleled that in cyanide‐resistant respiration activity, suggesting that the 39 kDa protein is the precursor of the 36 kDa protein, which is responsible for cyanide‐resistant respiration.