Premium
Nucleotide sequence determination of chicken glucagon precursor cDNA
Author(s) -
Hasegawa Shin,
Terazono Kimio,
Nata Koji,
Takada Tatsuyuki,
Yamamoto Hiroshi,
Okamoto Hiroshi
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80779-i
Subject(s) - complementary dna , glucagon , peptide sequence , peptide , signal peptide , amino acid , cdna library , nucleic acid sequence , biology , biochemistry , microbiology and biotechnology , chemistry , gene , hormone
cDNA clones coding for glucagon were isolated from a chicken pancreas cDNA library, and the nucleotide and amino acid sequences were determined. The amino acid sequence of chicken glucagon was HSQGTFTSDYSKYLDSRRAQDFVQWLMST, which was contained in the 151‐amino acid long precursor, being preceded by a signal sequence and an amino‐terminal peptide (NH 2 ‐peptide) and followed by an intervening peptide and a glucagon‐like peptide I (GLP‐I). Chicken preproglucagon, however, lacked GLP‐II and intervening peptide II which have been shown to be contained in mammalian glucagon precursors.