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The GC‐selective ligand mithramycin alters the structure of (AT) n sequences flanking its binding sites
Author(s) -
Cons Benjamin M.G.,
Fox Keith R.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80775-e
Subject(s) - chemistry , ligand (biochemistry) , stereochemistry , biophysics , biochemistry , receptor , biology
DNA fragments containing (AT) n inserts cloned adjacent to putative mithramycin binding sites have been examined by footprinting experiments using a variety of nucleases in the presence of the drug. The results demonstrate that mithramycin induces a DNA structural change which renders adjacent (AT) n sequences sensitive to attack by DNase II. Significant changes are also revealed with DNase I and micrococcal nuclease. The results are consistent with a model in which mithramycin opens the DNA minor groove changing it to a structure which is locally more like A‐DNA.