Premium
High concentrations of low density lipoprotein decrease basement membrane‐associated heparan sulfate proteoglycan in cultured endothelial cells
Author(s) -
Olgemöller Bernhard,
Schleicher Erwin D.,
Schwaabe Susanne,
Guretzki Hans-Joachim,
Gerbitz Klaus D.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80758-b
Subject(s) - fibronectin , basement membrane , chemistry , proteoglycan , heparan sulfate , perlecan , extracellular , low density lipoprotein , extracellular matrix , lipoprotein , biochemistry , medicine , endocrinology , microbiology and biotechnology , cholesterol , biology , glycosaminoglycan
The effect of increasing low density lipoprotein (LDL) concentrations on the synthesis of basement membrane components was investigated in proliferating porcine aortic endothelial cells (PAEC) in culture. Basement membrane‐associated heparan sulfate proteoglycan (HSPG) and fibronectin were determined by enzyme immunoassay. Low extracellular LDL‐levels increase, high extracellular LDL‐levels decrease the HSPG content of PAEC. Fibronectin synthesis was only slightly affected while proliferation and metabolic activity as assessed by lactate production were constant. Insulin or high extracellular glucose did not influence the effect of LDL on basement membrane components.