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Evidence that chlorpromazine and prostaglandin E 1 but not neomycin interfere with the inositol phospholipid metabolism in intact human platelets
Author(s) -
Tysnes Ole-Bjørn,
Steen Vidar M.,
Frølich Karin W.,
Holmsen Holm
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80757-a
Subject(s) - neomycin , phosphatidylinositol , inositol , chlorpromazine , phospholipid , phosphatidic acid , platelet , chemistry , metabolism , second messenger system , biochemistry , phospholipase c , endocrinology , signal transduction , biology , medicine , receptor , membrane , antibiotics
Human platelets that had been prelabelled with [ 32 P]P i were stimulated with trombin in the presence or absence of neomycin, prostaglandin E 1 (PGE 1 ) or chlorpromazine. The content of [ 32 P]P i in phosphatidylinositol 4‐phosphate, phosphatidylinositol 4,5‐bisphosphate and phosphatidic acid (PA) were determined. The data demonstrate that PGE 1 and chlorpromazine but not neomycin interfere with the tight metabolic relationship that exists between the inositol phospholipids and PA in thrombin‐stimulated platelets [(1989) Biochem. J. 263, 621‐624]. Our results therefore indicate that neomycin does not inhibit signal transduction in intact platelets at the level of the inositol phospholipid metabolism.