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In vitro protein translocation into inverted membrane vesicles prepared from Vibrio alginolyticus is stimulated by the electrochemical potential of Na + in the presence of Escherichia coli SecA
Author(s) -
Tokuda Hajime,
Kim Young Jae,
Mizushima Shoji
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80751-4
Subject(s) - vibrio alginolyticus , chromosomal translocation , escherichia coli , vesicle , twin arginine translocation pathway , vibrio , in vitro , microbiology and biotechnology , biology , chemistry , membrane , biochemistry , membrane protein , bacteria , membrane transport protein , gene , genetics
A protein translocation system was reconstituted from inverted membrane vesicles prepared from Na + pump‐possessing Vibrio alginolyticus and purified Escherichia coli SecA. The translocation required ATP and was stimulated by the functioning of the Na + pump, suggesting that the electrochemical potential of Na + but not that of H + , is important for protein translocation in Vibrio .