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Expression of voltage‐gated K + channels in insulin‐producing cells
Author(s) -
Betsholtz Christer,
Baumann Arnd,
Kenna Susan,
Ashcroft Frances M.,
Ashcroft Stephen J.H.,
Berggren Per-Olof,
Grupe Andrew,
Pongs Olaf,
Rorsman Patrik,
Sandblom John,
Welsh Michael
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80719-y
Subject(s) - insulin , coding region , microbiology and biotechnology , cell culture , polymerase chain reaction , biology , islet , messenger rna , rna , gene , chemistry , genetics , endocrinology
We have used the polymerase chain reaction (PCR) with primers against the S5 and S6 regions of voltage‐gated K + channels to identify 8 different specific amplification products using poly(A) + RNA isolated from islets of Langerhans from obese hyperglycémic ( ob/ob ) mice and from the two insulin‐producing cell lines HIT T15 and RINmSF. Sequence analysis suggests that they derive from mRNAs coding for a family of voltage‐gated K + channels; 5 of these have been recently identified in mammalian brain and 3 are novel. These hybridize in classes to different mRNAs which distribute differently to a number of tissues and cell lines including insulin‐producing cells.