Premium
DNA methylation in Trypanosoma cruzi
Author(s) -
Rojas M.Véronica,
Galanti Norbel
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80717-w
Subject(s) - trypanosoma cruzi , dna , biology , restriction enzyme , microbiology and biotechnology , dna methylation , methylation , nuclear dna , 5 methylcytosine , biochemistry , gene , parasite hosting , gene expression , world wide web , computer science , mitochondrial dna
DNA isolated from the protozoan Trypanosoma cruzi has been found to contain 5‐methylcytosine. Analysis of T. cruzi DNA by both Hpa II and Msp I restriction endonucleases suggests that the sequence ‐CCGG‐ is not methylated. Probably T. cruzi DNA also contains N 6 ‐methyladenine. This report constitutes the first clear demonstration of the presence of methylated bases in the nuclear DNA from trypanosomes.