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Isolation and RNA sequence analysis of cytochrome b mutants resistant to funiculosin, a center i inhibitor of the mitochondrial ubiquinol‐cytochrome c reductase in Saccharomyces cerevisiae
Author(s) -
di Rago J.-P.,
Perea J.,
Colson A.-M.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80713-s
Subject(s) - cytochrome b , cytochrome c1 , coenzyme q – cytochrome c reductase , biochemistry , ubiquinol , cytochrome , mutant , mitochondrial respiratory chain , cytochrome p450 reductase , saccharomyces cerevisiae , cytochrome c , biology , yeast , chemistry , microbiology and biotechnology , enzyme , mitochondrion , mitochondrial dna , gene
Funiculosin is a well‐known inhibitor of the mitochondrial respiratory chain, probably acting at the ubiquinone reducing site or center i of QH 2 cytochrome c reductase. We report here the isolation, mapping and RNA sequence analysis of yeast apo‐cytochrome b mutants resistant to this inhibitor. Funiculosin‐resistance was found to be conferred, in 4 independent isolates, upon replacement of a leucine residue by phenylalanine in position 198 of the cytochrome b polypeptide chain.