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Tetanus toxin‐induced protein kinase C activation and elevated serotonin levels in the perinatal rat brain
Author(s) -
Aguilera Jose,
Lopez Lluis A.,
Yavin Ephraim
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80705-n
Subject(s) - protein kinase c , serotonin , pargyline , serotonergic , monoamine oxidase , endocrinology , chemistry , pharmacology , medicine , cytosol , tetanus , phosphatidylserine , kinase , biology , receptor , biochemistry , enzyme , immunology , phospholipid , membrane , vaccination
A single intracerebral injection of tetanus toxin (TeTox) is able to produce a time‐dependent translocation of Ca 2+ ‐phosphatidylserine‐dependent protein kinase C (PKC) in close‐to‐tenn rat brain. TeTox‐triggered translocation of PKC is dose‐ and time‐dependent, can be prevented by tetanus antitoxin, and does not occur upon administration of toxin fragments B and C. TeTox‐triggered PKC translocation is accompanied by a timedependent increase in brain serotonin (5‐HT). Increase of brain 5‐HT is independent of monoamine oxidase inhibition by pargyline. Phorbol ester and TeTox cause a significant increase in serotonin while H‐7, a kinase inhibitor, does not affect serotonin levels but abolishes the effect of TeTox. Gangliosides prevent TeTox‐triggered 5‐HT increase. The data are consistent with the possibility that TeTox acts effectively on the serotonergic innervation, presumably in conjunction with PKC to cause accumulation of serotonin.