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Cloning and sequencing of the gene encoding cytochrome c ‐551 from Pseudomonas aeruginosa
Author(s) -
Arai Hiroyuki,
Sanbongi Yoshihiro,
Igarashi Yasuo,
Kodama Tohru
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80669-a
Subject(s) - biology , gene , microbiology and biotechnology , molecular cloning , oligonucleotide , sequence analysis , peptide sequence , nucleic acid sequence , cytochrome , cytochrome c , genetics , biochemistry , enzyme , mitochondrion
The cytochrome c ‐551 gene from Pseudomonas aeruginosa was cloned by using two oligonucleotide probes, which had been synthesized based on the known primary structure of the protein. The restriction map of the cloned DNA and sequence analysis showed that the cytochrome c ‐551 gene is located 50 bp downstream of the nitrite reductase gene, which has recently been cloned and sequenced. DNA sequence analysis also indicated that cytochrome c ‐551 is synthesized in vivo as a precursor having an amino‐terminal signal sequence consisting of 22 amino acid residues.