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Simultaneous recordings of glucose dependent electrical activity and ATP‐regulated K + ‐currents in isolated mouse pancreatic β‐cells
Author(s) -
Smith Paul A.,
Ashcroft Frances M.,
Rorsman Patrik
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80667-8
Subject(s) - tolbutamide , conductance , membrane potential , biophysics , chemistry , pancreatic islets , electrophysiology , endocrinology , medicine , biology , islet , insulin , physics , condensed matter physics
Membrane potential and membrane currents were recorded from single mouse pancreatic β‐cells using the perforated patch whole‐cell recording technique at 30 °C. Single β‐cells maintained in primary tissue culture exhibited glucose‐dependent electrical activity similar to that reported for freshly isolated intact islets. The resting input conductance (5.1±0.9 nS) was determined by ATP‐regulated K + (K ATP ) channels as it was blocked by 1 mM tolbutamide. 8 mM glucose decreased the input conductance by 80%. The input conductance at −70 mV was of a similar value during the plateau phase and during the silent phase of electrical activity in 8 mM glucose. This suggests that oscillations of K ATP channel activity do not underlie the slow waves.