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Presence of immunoreactive endothelin in human milk
Author(s) -
Lam Hing-Chung,
Takahashi Kazuhiro,
Ghatei Mohammed A.,
Bloom Stephen R.
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80666-7
Subject(s) - fast protein liquid chromatography , radioimmunoassay , chromatography , size exclusion chromatography , endothelins , endothelin receptor , chemistry , high performance liquid chromatography , biochemistry , receptor , enzyme
Endothelin‐like immunoreactivity was detected in human milk at a concentration of 6.8 ± 1.6(mean ± SEM; n = 16) using a highly sensitive radioimmunoassay. Gel filtration and fast protein liquid chromatography (FPLC) verified the identity of the endothelin. FPLC revealed 4 peaks, one eluting just after the void volume, and the other three in the positions of endothelin‐1, ‐2, and ‐3, respectively.