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Group II phospholipase A 2 mRNA synthesis is stimulated by two distinct mechanisms in rat vascular smooth muscle cells
Author(s) -
Nakano Tohru,
Ohara Osamu,
Teraoka Hiroshi,
Arita Hitoshi
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80663-4
Subject(s) - lipopolysaccharide , tumor necrosis factor alpha , vascular smooth muscle , messenger rna , phospholipase a2 , endocrinology , inflammation , secretion , phospholipase c , medicine , pathogenesis , phospholipase , enzyme , interleukin , phospholipase a , chemistry , biology , cytokine , biochemistry , smooth muscle , gene
Two potent inflammatory mediators, interleukin 1 (IL‐1) and tumor necrosis factor (TNF) as well as lipopolysaccharide (LPS) increased group II phospholipase A 2 (PLA 2 ) mRNA levels, which resulted in enhanced secretion of the PLA 2 enzyme from rat smooth muscle cells. cAMP‐elevating agents also stimulated the release of PLA 2 and increased the mRNA, but IL‐1, TNF and LPS did not affect cAMP levels. Furthermore, the effects of TNF and cAMP‐elevating agents were not additive but synergistic. Therefore, we concluded that the level of rat group II PLA 2 mRNA is controlled at least by two distinct mechanisms, one involves cAMP and the other is mediated by TNF, IL‐1 and LPS. This study also suggests important roles of group II PLA 2 in pathogenesis of vascular inflammation.