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Sphingomyelin is synthesized in the cis Golgi
Author(s) -
Jeckel Dieter,
Karrenbauer Achim,
Birk Rolf,
Richard Schmidt R.,
Wieland Felix
Publication year - 1990
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(90)80659-7
Subject(s) - sphingomyelin , golgi apparatus , ceramide , organelle , biochemistry , sphingosine , chemistry , sphingolipid , membrane , enzyme , microbiology and biotechnology , biology , endoplasmic reticulum , apoptosis , receptor
We have employed in vitro a truncated ceramide analogue with 8 carbon atoms in the sphingosine and the fatty acyl residue, each, to investigate the activity of various membrane fractions to synthesize truncated sphingomyelin. This shortened ceramide readily diffuses through membranes and therefore can easily find access to the lumina of intact organelles. Sphingomyelin synthase activity resides in the Golgi apparatus, and after sucrose density gradient centrifugation of Golgi‐enriched fractions sphingomyelin synthesis follows a cis Golgi marker enzyme.